IN VITRO EVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF ALUM AND ITS BHASMA (SPHATIKA BHASMA)

To overcome the burden of antimicrobial resistance, greater attention has been paid to activity screening from natural products. One such products used for therapeutic purposes is Alum. Besides, Ayurvedic Bhasma formulations have treatment various ailments thousands years all over world and widely in India. Hence, present vitro study, Alum as well its Bhasma- Sphatika was evaluated against total 10 organisms, viz., 4 gram negative [Escherichia coli (ATCC-10148), Klebsiella pneumoniae (ATCC-700603), Pseudomonas aeruginosa (Fishers Immuno Type IV), Salmonella typhi (NCTC-786)], positive [Bacillus subtilis (ATCC-9372), Enterococcus faecalis (ATCC-29212), Methicillin Resistant Staphylococcus aureus (MRSA ATCC-25923), Sarcina lutea (NCTC-2470)] 2 fungi [Aspergillus niger (ATCC-16404) Candida albicans (ATCC-10231)] using assays MIC, MBC/MFC Agar Well Diffusion method. In general, MIC values were lower than that revealed broad spectrum antibacterial activities inhibiting organisms moderate antifungal activities. Furthermore, different combinations prepared (AL), (SB) standard antibiotics subjected determine their combinatorial effect Assay. The (AL + CP), (SB CP) SB displayed synergistic P. aeruginosa. Antimicrobial Antioxidant potential could be attributed Phenolic content. Overall, exhibited better